• Macromolecules-indexes-Serumwerk

• M01 – Preparation of gradient solutions (for macromolecules and macromolecular complexes)

• M02 – Preparation of discontinuous and continuous gradients (for macromolecules and macromolecular complexes)

• M03 – Self-generated gradients (macromolecules/macromolecular complexes)

• M04 – Harvesting gradients

• M05 – Analysis of gradients

• M06 – Isolation of plasmid DNA in self-generated gradients of iodixanol using DAPI as a fluorescent marker

• M07 – Analysis of mammalian and non-mammalian HDL, LDL and VLDL

• M08 – Fractionation of plasma lipoprotein sub-classes

• M09 – Analysis of protein complex formation, microtubules and cytoskeleton

• M10 – Separation of proteoliposomes from proteins and liposome-encapsulated macromolecules from liposomes

• M11 – Analysis of protein size in pre-formed gradients

• M12 – Analysis of proteins in small volume self-generated gradients

• M13 – Fractionation of nucleic acids and nucleic acid-protein complexes

OPTIPREP™ APPLICATION SHEET INDEX

MACROMOLECULES & MACROMOLECULAR COMPLEXES

• The Index is divided into two sections: General methods for preparing gradients and analysing gradients (A).
• An alphabetical macromolecule/macromolecular complex type index (B).
• To open an Application Sheet click on the relevant [Application Sheet M–]

A. GENERAL METHODS

Preparation of gradient solutions [Application Sheet M01]
Preparation of discontinuous and continuous gradients [Application Sheet M02]
Preparation of self-generated gradients [Application Sheet M03]
Harvesting gradients [Application Sheet M04]
Analysis of gradients [Application Sheet M05]

B. MACROMOLECULE/MACROMOLECULAR COMPLEX INDEX

Cytoskeleton [Application Sheet M09]
Liposome-encapsulated macromolecules [Application Sheet M10]
Microtubules [Application Sheet M09]
Mysoin II [Application Sheet M09]
Nucleic acids [Application Sheet M13]
Nucleic acid-protein complexes [Application Sheet M13]
Plasma lipoproteins
   HDL, LDL, VLDL analysis [Application Sheet M07]
   Lipoprotein subclass analysis [Application Sheet M08]
Plasmid DNA purification [Application Sheet M06]
Prion proteins [Application Sheet M09]
Proteins
   Complex formation analysis [Application Sheet M09]
   Mitochondrial proteins (see “Nucleic acid-protein complexes”)
   Size analysis in pre-formed gradients [Application Sheet M11]
   Size analysis in self-generated gradients [Application Sheet M12]
Proteoliposomes (separation from proteins) [Application Sheet M10] 

OptiPrep™ Macromolecule and Macromolecular 

Complex Application Sheet Index March 2020

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