• Virus-index-Serumwerk

• V01 – Preparation of density gradient solutions

• V02 – Preparation of discontinuous and continuous gradients

• V03 – Preparation of self-generating gradients

• V04 – Harvesting gradients

• V05 – Analysis of gradients

• V06 – Purification of viruses and viral vectors using OptiPrep™

• V07 – Purification of Group I (ds)DNA viruses: adenovirus and removal of helper virus

• V08 – Purification of Group I (ds)DNA viruses: Herpesviridae and Baculoviridae in a self-generated gradient

• V09 – Purification of Group I (ds)DNA viruses: Herpesviridae and Asfaviridae in a pre-formed gradient

• V10 – Purification of Group I (ds)DNA viruses: human papillomavirus

• V11 – Purification of Group I (ds)DNA viruses: Polyomaviridae

• V12 – Purification of Group I (ds)DNA viruses: Poxviridae

• V13 – Purification of Group I (ds)DNA viruses (non-mammalian sources): Iridoviridae

• V14 – Purification of Group II (ss)DNA viruses: Parvoviridae: recombinant adenoassociated virus (rAAV) and avian AAV

• V15 – Purification of Group II (ss)DNA viruses: Protoparvovirus – murine minute virus

• V16 – Purification of Group II (ss)DNA viruses: Parvovirinae – parvovirus

• V17 – Purification of Group III (ds)RNA viruses: Reoviridae

• V18 – Purification of Class IV ((+)ssRNA) viruses: Caliciviridae family

• V19 – Purification of Group IV ((+)ss) RNA viruses: Flaviviridae family and hepatitis E in pre-formed gradients

• V20 – Purification of Group IV ((+)ss) RNA viruses: Flaviviridae family: hepatitis C particles in a self-generated gradient

• V21 – Purification of Group IV ((+)ss) RNA viruses: Nidovirales: Coronaviridae and Arteriviridae

• V22 – Purification of Group IV ((+)ss) RNA viruses: Togaviridae: Alphavirus and Rubivirus

• V23 – Purification of Group V ((-)ss) RNA viruses: Arenaviruses: Lassa virus, Tacaribe virus, Junin virus, Lymphocytic choriomeningitic virus (LCV)

• V24 – Purification of Group V ((-)ss) RNA viruses: Bunyaviridae – Hantavirus, Orthobunyavirus and Phlebovirus

• V25 – Purification of Group V ((-)ss) RNA viruses: Ebola virus capsid assembly analysis and purification of Ebola pseudovirus in self-generated gradients

• V26 – Purification of Group V ((-)ss) RNA viruses: Orthomyxoviridae

• V27 – Purification of Group V ((-)ss) RNA viruses: Rhabdoviridae: Rabies virus (Lyssavirus) and vesicular stomatitis virus

• V28 – Purification of Group V ((-)ss) RNA viruses: Paramyxoviridae – Paramyxovirinae and Pneumovirinae

• V29 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Alpharetrovirus: Rous sarcoma virus

• V30 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Betaretrovirus: Mason-Pfizer Monkey virus

• V31 – Purification of Group VI (ss)RNA-RT viruses: Retroviridae: Deltaretrovirus, human T-cell lymphotropic virus (HTLV-1) and human endogenous retrovirus

• V32 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Gammaretrovirus: murine oncornavirus

• V33 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Gammaretrovirus: Moloney murine leukemia virus

• V34 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Lentivirus: Human immunodeficiency virus – 1 (HIV-1) and lentivirus vectors

• V35 – Purification and analysis of Group VI (ss)RNA-RT viruses: Retroviridae: Spumaretrovirinae: Foamy viruses

• V36 – Purification and analysis of Group VII (ds RNA-RT): Hepadnaviridae: Orthohepadnavirus: hepatitis B virus

• V37 – Purification of viruses from non-mammalian cells and tissues

• V38 – Purification and analysis of bacteriophages

OPTIPREP™ VIRUS APPLICATION SHEET INDEX

• All of the general information on gradient production and analysis plus pre-gradient virus concentration are contained in Application Sheets V01-V05.
• V06 summarizes the range of virus purification strategies
• All the virus gradient protocols (V07-V36) have been listed according to the seven groups of the Baltimore Virus Classification scheme.
• Within each group, Families (sometimes preceded by the Order name), and where appropriate Subfamilies, are listed alphabetically in italics. Within each Family or Subfamily individual viruses are also listed alphabetically.
• In some cases more than one Application Sheet for a specific virus type may be provided, if significantly different practical strategies are available.
• Protocols for one particular virus in a Group may be applicable to other viruses in the same group with a similar overall structure and composition, but this can only be determined experimentally.
• Yeast retrotransposons, although not part of the Baltimore Virus Classification system, are considered similar in structure to retroviruses. They are included in Group VI listings.
•  Plant Virus (V37) and Bacteriophage (V38) Application Sheets can accessed from this index after the entry for Group VII viruses.
• To access the Application Sheet(s) referred to in the list: return to the 2020Virapp file and select the appropriate V number.

1. Gradient preparation, virus concentration and gradient analysis

Preparation of density gradient solutions [Application Sheet V01]
Preparation of discontinuous and continuous gradients [Application Sheet V02]
Preparation of self-generated gradients [Application Sheet V03]
Harvesting gradients [Application Sheet V04]
Analysis of gradients [Application Sheet V05]

2. Virus purification strategies (a review) [Application Sheet V06]

3. Virus purification

Group I (ds)DNA viruses

Adenoviridae
Adenovirus [Application Sheet V07]
Helper-dependent virus, separation from [Application Sheet V07]
Asfaviridae
African swine fever virus [Application Sheet V09]
Baculoviridae
Baculovirus vectors [Application Sheet V08]
Herpesviridae
Cytomegalovirus
Self-generated gradient [Application Sheet V08]
Pre-formed gradient [Application Sheet V09]
Epstein-Barr virus
Self-generated gradient [Application Sheet V08]
Pre-formed gradient [Application Sheet V09]
Herpesviridae (contd)
Herpes simplex virus
Self-generated gradient [Application Sheet V08]
Pre-formed gradient [Application Sheet V09]
Herpes virus vectors [Application Sheet V08]
Rhadinovirus [Application Sheet V09]
Iridoviridae
Iridovirus [Application Sheet V13]
Papillomaviridae
Human papillomavirus [Application Sheet V10]
Phycodnaviridae
Chlorovirus [Application Sheet V13]
Polyomaviridae
BK virus [Application Sheet V11]
JC virus [Application Sheet V11]
Merkel cell carcinoma virus [Application Sheet V11]
Simian virus 40 (SV40) [Application Sheet V11]
Poxviridae
Molluscipoxvirus [Application Sheet V12]
Vaccinia virus [Application Sheet V12]

Group II (ss)DNA viruses

Anelloviridae
Torque teno virus [Application Sheet V15]
Parvoviridae
Densovirinae
Dependovirus
Avian adeno-associated virus [Application Sheet V14]
Recombinant adeno-associated virus [Application Sheet V14]
Parvovirinae
Minute virus of mice [Application Sheet V15]
Parvovirus [Application Sheet V16]

Group III (ds)RNA viruses

Reoviridae
Birnavirus
Espirito Santo virus [Application Sheet V17]
Sedoreovirinae
Rotavirus [Application Sheet V17]
Seadornavirus [Application Sheet V17]
Spinareovirinae
Dinovernavirus [Application Sheet V17]

Group IV ((+)ss)RNA viruses

Caliciviridae
Lagovirus
Rabbit haemorrhagic fever virus [Application Sheet V18]
Norovirus
Norwalk virus [Application Sheet V18]
Flaviviridae
Flavivirus
Dengue virus [Application Sheet V19]
West Nile virus [Application Sheet V19]
Yellow fever virus [Application Sheet V19]
Hepacivirus
Hepatitis C virus
Pre-formed gradient [Application Sheet V19]
Self-generated [Application Sheet V20]
Hepevirus
Hepatitis E virus [Application Sheet V19]
Pestivirus
Bovine diarrhea virus [Application Sheet V19]
Nidovirales – Arteriviridae
Porcine reproductive & respiratory syndrome virus [Application Sheet V21]
Nidovirales – Coronaviridae
SARS coronavirus [Application Sheet V21]
Togaviridae
Alphavirus
Chikungunya virus [Application Sheet V22]
Semliki Forest virus [Application Sheet V22]
Sindbis virus [Application Sheet V22]
Venezuelan equine encephalitis virus [Application Sheet V22]
Rubivirus
Rubella virus [Application SheetV22]
Tombusviridae
Dianthovirus
Red clover mosaic virus [Application Sheet V22]

Group V ((-)ss) RNA viruses

Arenaviridae
Arenavirus
Lassa virus [Application Sheet V23]
Tacaribe virus [Application Sheet V23]
Bunyaviridae
Hantavirus
Hantavirus [Application Sheet V24]
Orthobunyavirus
Bunyamweravirus [Application Sheet V24]
Phlebovirus
Rift valley fever virus [Application Sheet V24]
Filoviridae
Ebola virus [Application Sheet V25]
Orthomyxoviridae
Influenza virus [Application Sheet V26]
Rhabdoviridae
Lyssavirus
Rabies virus [Application Sheet V27]
Vesiculovirus
Vesicular stomatitis virus [Application Sheet V27]
Paramyxoviridae
Paramyxovirinae
Avulavirus
Newcastle disease virus [Application Sheet V28]
Morbillivirus
Measles virus [Application Sheet V28]
Respirovirus
Swine paramyxovirus [Application Sheet V28]
Pneumovirinae
Human respiratory syncytial virus [Application Sheet V28]

Group VI (ss)RNA-RT viruses

Retroviridae
Alpharetrovirus
Rous sarcoma virus [Application Sheet V29]
Betaretrovirus
Mason-Pfizer monkey virus (Simian retrovirus) [Application Sheet V30]
Deltaretrovirus
Human T cell lymphotropic virus [Application Sheet V31]
Gammaretrovirus
Moloney murine leukaemia virus [Application Sheet V33]
Murine oncornavirus [Application Sheet V32]
Lentivirus
Feline immunodeficiency virus [Application Sheet V34]
Human immunodeficiency virus [Application Sheet V34]
Spumaretroviridae
Spumaviridae
Foamy virus [Application Sheet V35]
Yeast retrotransposons [Application Sheet V37]

Group VII (ds)RNA-RT

Hepadnaviridae
Orthohepadnavirus
Hepatitis B [Application Sheet V36]

Plant viruses [Application Sheet V37]
Bacteriophages [Application Sheet V38]

OptiPrep™ Virus Application Sheet Index February 2020

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