一般描述
Gey′s平衡盐溶液/Gey's Balanced Salt Solution (GBSS)是一种磷酸盐碳酸氢盐缓冲细胞悬浮液和洗涤液。其是一种完整的平衡盐溶液,具有不同“盐形式”的独特组合。其含有与EBSS、HBSS和DPBS相同的五种基本离子。
应用
Gey平衡盐溶液已用于:
- 器官型小鼠脑切片培养[1]
- 小鼠原代肝窦内皮细胞的分离(LSEC)[2]
- 器官型海马切片培养[3]
参考资料
- Chang TC, Mikheev AM, Huynh W, Monnat RJ, Rostomily RC, Folch A. Parallel microfluidic chemosensitivity testing on individual slice cultures. Lab Chip. 2014 Dec 7;14(23):4540-51. doi: 10.1039/c4lc00642a. Epub 2014 Oct 2. PMID: 25275698; PMCID: PMC4217250.
- Olsavszky V, Sticht C, Schmid CD, Winkler M, Wohlfeil SA, Olsavszky A, Schledzewski K, Géraud C, Goerdt S, Leibing T, Koch PS. Exploring the transcriptomic network of multi-ligand scavenger receptor Stabilin-1- and Stabilin-2-deficient liver sinusoidal endothelial cells. Gene. 2021 Feb 5;768:145284. doi: 10.1016/j.gene.2020.145284. Epub 2020 Oct 31. PMID: 33130055.
- Nowacka A, Borczyk M, Salamian A, Wójtowicz T, Włodarczyk J, Radwanska K. PSD-95 Serine 73 phosphorylation is not required for induction of NMDA-LTD. Sci Rep. 2020 Feb 6;10(1):2054. doi: 10.1038/s41598-020-58989-2. PMID: 32029829; PMCID: PMC7005143.