人  单个核细胞  淋巴细胞  骨髓  脐带血  外周血  

Histopaque-1077 Hybri-Max

品牌：Sigma/西格玛点击下载说明书

储存：Stored at 2-8 °C protected from light

sterility：sterile-filtered

Quality Level：400

product line：BioReagent

form：liquid

technique(s)：cell culture | hybridoma: suitable         single cell analysis: suitable

impurities：endotoxin, tested

density：1.077 g/cm³ at 25 °C (lit.)

storage temp.  2-8°C

一般描述

Histopaque^®-1077是包含多蔗糖和泛影酸钠的溶液，密度调整到1.077 +/- 0.001 g/cm³。[1]这一培养基帮助来自小体积全血的活的淋巴细胞快速恢复。这一技术适合于调节淋巴溶解和人类淋巴细胞抗原（HLA）²分型的细胞。它还可用作T-、B-和“空”淋巴细胞计数前的起始分离步骤。用来建立密度培养基，用于淋巴细胞和其他单核细胞的纯化。

应用

Histopaque^®-1077 Hybri-Max^™已用于分离：

• 外周血单个核细胞（PBMC）与肝素化血，梯度离心法[2]
• 来自外周血的免疫细胞（淋巴细胞）[3]
• PBMC与血液[4]

General description

Histopaque^®-1077 is a solution comprising polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/cm³. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell-mediated lympholysis and human lymphocyte antigen (HLA)² typing. It may also be employed as the initial isolation step before the enumeration of T-, B-, and null lymphocytes. It is used to create a density medium for the purification of lymphocytes and other mononuclear cells.

Application

Histopaque^®-1077 Hybri-Max^™ has been used to isolate:

• peripheral blood mononuclear cells (PBMCs) from heparinized blood by gradient centrifugation
• immune cells (lymphocytes) from peripheral blood
• PBMCs from blood

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC